The Kyiv Protocol on Pollutant Release and Transfer Registers became international law binding its Parties on 8 October 2009. It is the only legally binding international instrument on pollutant release and transfer registers. Manifesto (short version) Short Version. For all their concerns with global competitiveness, migration and terrorism, only one prospect truly terrifies the Powers of Europe: Democracy! DOWNLOAD SHORT VERSION. A MANIFESTO FOR DEMOCRATISING EUROPE.

The Kyiv Protocol on Pollutant Release and Transfer Registers became international law binding its Parties on 8 October 2009. It is the only legally binding international instrument on pollutant release and transfer registers. Its objective is 'to enhance public access to information through the establishment of coherent, nationwide pollutant release and transfer registers (PRTRs).' PRTRs are inventories of pollution from industrial sites and other sources. Although regulating information on pollution, rather than pollution directly, the Protocol is expected to exert a significant downward pressure on levels of pollution, as no company will want to be identified as among the biggest polluters. All UN Member States can join the Protocol, including those which have not ratified the Aarhus Convention and those which are not members of the United Nations Economic Commission for Europe. It is by design an 'open' global treaty.

Virion-based ELISA Polystyrene 96-well microtiter plates (MaxiSorp, Nunc) were coated with purified CHIKV (20,000 infectious virions per µl in PBS; 50 µl per well). Blots were exposed to films (Pierce, Thermo Scientific) and developed. Wells were blocked with PBS containing 0.05% Tween-20 and 5% non-fat milk (PBST-milk), and plates were incubated for 1.5 hours at 37°C.